Dados do Trabalho

Título

ORAL CANCER CELLS SECRETE THE NEUROTRANSMITTER NOREPINEPHRINE

Introdução

Studies from our research group and others have described the involvement of these neurohormones in oral squamous cells carcinoma (OSCC) progression. Despite knowledge regarding the impact of catecholamines on oral cancer progression, there has no investigation whether oral cancer cells are able to secrete stress-related hormones.

Objetivo

The current study aimed to investigate the autocrine produce of NE by keratinocytes (Hacat) and OSCC-derived cell lines (SSC9 and SCC25). The presence of NE in culture medium and its correlation with the presence of Fetal Bovine Serum (FBS) was also investigated.

Métodos

NE levels were measured by enzyme-linked immunosorbent assay (ELISA) in the culture media without cells supplemented with normal (10%) or reduced FBS concentration (0.5%). In the culture medium without cells, NE levels were correlated to FBS concentrations.

Resultados

The medium with highest FBS concentration (10%) had higher hormone levels than those with reduced FBS concentrations (0.5%) (p < 0.05). In culture media with cultured cell lines, the supernatants from all cell lines showed higher NE levels compared to the culture medium without cells. When the cell supernatant was compared to its respective culture media in basal conditions (without cells), the cancer cells secreted higher NE levels in the supernatant compared to HaCaT cells (14.9 times for SCC9 cells and 15.1 times for SCC25 cells).

Conclusões

In the current study, we demonstrated for the first time that OSCC cells secrete the neurotransmitter NE. Further studies are required to understand the role of autocrine catecholamines secretion in the oral cancer progression.

Palavras-chave

Norepinephrine, squamous cells carcinoma, autocrine growth factors.

Financiador do resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (Processo: 13/24661-6).