Dados do Trabalho

Título

Characterization of gene expression in dogs with cutaneous and multicentric lymphoma with the RNA-seq technique

Introdução

Lymphoma is a neoplastic disease characterized by clonal proliferation of malignant lymphocytes that originates from bone marrow, thymus, spleen, liver or lymph nodes. However, it can develop in any organs by continuous migration of lymphocytes to different organs in the body. Lymphoma is among the most common malignant tumors in dogs, representing 8.5 to 9% of all tumors in this specie, wherein the multicentric presentation is the most frequent. Cutaneous lymphoma is considered rare, representing 3 to 8% of all lymphomas clinical presentation and instead to multicentric lymphomas, the cutaneous form is unresponsive to antineoplastic chemotherapy. Recently, gene expression studies are essential to bring new discoveries in cancer research. The RNA-seq technique can identify new genes, including low expression genes.

Objetivo

Thus, this study aims to evaluate gene expression profile and identify differentially expressed genes in multicentric and cutaneous canine lymphomas using the RNA-seq technique.

Métodos

Samples were collected from 16 animals at the Veterinary Hospital “Governador Laudo Natel” of FCAV/UNESP, Jaboticabalem Campus. The experiment was designed in 2 groups, composed by the control group (2 animals) and the neoplasm group (14 animals). The first analysis performed was the extraction of RNAseq data to identify interactions. Subsequently, the construction of RNA libraries and RNA sequencing of Multicentric and Cutaneous Lymphomas was carried out. To compose the analyses, differential expression analyzes were performed between the genes to identify predictive biomarkers.

Resultados

A total of 270 genes were identified in the performed analyses. Among them, 28S ribosomal RNA and CPLX3 (complexin 3) and showed a significant increase in the neoplasia group when compared to the control group.

Conclusões

With the results, 28S ribosomal RNA and CPLX3 genes that can be used as prognostic and predictive markers, comparing it between cutaneous and multicentric clinical presentations of lymphomas in dogs.

Palavras-chave

RNA_Seq, lymphoma, dog